RNA

Part:BBa_K5142008:Design

Designed by: Yuchen Liang   Group: iGEM24_HunanU   (2024-09-27)


amber suppressor tRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1137
    Illegal SpeI site found at 15
    Illegal PstI site found at 1556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1543
    Illegal EcoRI site found at 1137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1137
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1137
    Illegal SpeI site found at 15
    Illegal PstI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1137
    Illegal SpeI site found at 15
    Illegal PstI site found at 1556
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure the synthesis efficiency of A27L-3stop, the DNA fragment of the amber suppressor tRNA that we provide includes nine sequences that can express the amber suppressor tRNA.


Source

This part was synthesized according to the sequence of the amber suppressor tRNA gene in E.coli genome .(Gene ID: 945269)

References